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CONSULTANCY >> SOME TYPICAL PROJECTS >>
PROTOTYPE FLUORIMETER
 
high intensity mercury lamp photomultiplier detector concave mirror optics
 
 
CLIENT A UK manufacturer of clinical chemistry analysers.
   
PROBLEM
To design the illumination and detection systems for a fluorescence based HPLC analyser.
 
 
SOLUTION
The solution required a number of stages because of the extremely low level of the excited fluorescence, which was not made clear at the outset.

The light source: A Xenon flash lamp, was tried but rejected because of high electrical noise, a high intensity mercury lamp gave excellent results.

The detector: As the amount of light excited was so small a photomultiplier had to be used, both standard and avalanche photodiodes proved insufficiently sensitive.

The optics: Given the constraints of the small space available and the necessity of focussing on a 1mm cross section flow cell, a system of concave mirrors was designed. The resulting system gave good signal levels on the full range of chemical samples.
 
 
TECHNOLOGIES REQUIRED
Photodetectors, Optics, Mechanical Engineering, Light Sources, Electronics.